Within Iribov diverse analytical techniques are applied to plants for breeding purposes or for quality control in production. The main product groups in the lab are flow cytometry and molecular testing via PCR methods.
The most important technique for the analytical lab is currently flow cytometry (FCM). The most common use of flow cytometry in our lab is for determination of the DNA amount, ploidy level of a plant, to determine aneuploidy or to detect interspecific hybrids.
The quality aspect of plants is becoming more and more important. However, pathogen infections do not always present with clear symptoms. PCR tests can be used as a tool to determine these infections. In 2010 Iribov started its own molecular laboratory for quality control in tissue culture plants using PCR.
Unfortunately, in some crops the DNA amount will not always give direct information of chromosome number of the plant, especially for hybrids from parents with different chromosome sizes.
The development of molecular techniques has led to some new approaches for plant breeding. However, the need for a molecular lab facility is a major barrier for many breeding companies looking to use these techniques.