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Flowcytometrie1 Flowcytometrie2

Flow cytometry

The most important technique for the analytical lab is currently flow cytometry (FCM). The most common use of flow cytometry in our lab is for determination of the DNA amount, ploidy level of a plant, to determine aneuploidy or to detect interspecific hybrids.

Flow cytometry is a technique where individual particles are passing in a flow along a measuring point where the particles are illuminated and the scattered light and fluorescence are measured. From a small amount of tissue (leaf or directly from seed) nuclei are isolated. These nuclei are stained with a DNA dye. The flow cytometer analyses the fluorescence intensity and with this the relative DNA amount. By using an internal standard it's possible to estimate the relative DNA content in a 1% range. With this accuracy conclusions can be drawn for many crops about the ploidy level of the plant, to determine aneuploidy or to detect interspecific hybrids.

It’s also possible to estimate pollen viability with flow cytometry.

Here you can download the order form Brassica aneuploidy.

Furthermore, Iribov is supplier for PARTEC flow cytometers and reagents.

 Analyse lab3 Analyse lab2